Aligning qPCR, dPCR, and NGS to unify and broaden the quantification revolution

Wednesday, September 29, 7 - 8:30 a.m.
Sponsored by Thermo Fisher Scientific
Hyatt Regency Atlanta, Regency Ballroom 6

Description

Polymerase Chain Reaction (PCR) technology, first discovered in 1985(1), laid the foundation for the modern and sophisticated methods of nucleic acid detection, quantification, and integration. Quantitative PCR (qPCR) and next generation sequencing (NGS) are among the most used molecular techniques for these purposes, and recent developments in digital PCR (dPCR) technology, which facilitate highly sensitive and precise absolute quantifications of nucleic acids, have accelerated its adoption in the field. Although typically compared and contrasted in terms of dynamic range, precision, and accuracy, qPCR, NGS, and dPCR are all positioned to answer a different breadth of molecular questions. In this session, we will explore the benefits of each technology and together how each strength can be leveraged to tell a more compelling and complete molecular story.

Reference:

1. Saikl R.K., Scharf S, Faloona F., Mullis K.B., Horn G.T, Erlich H. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985; 230:1350-1354 [PubMed][Google Scholar][Ref List]

Outcomes

  1. A technical overview of digital PCR as quantification tool
  2. How dPCR supplements qPCR and NGS

Moderator

Adam Langston
Sr. Manager
Thermo Fisher Scientific
Speaker

Paul Hung
General Manager & Senior Director of Digital PCR
Thermo Fisher Scientific