Detection of Infectious Disease at Species Resolution with Pathogen-derived Peptidomes

 

Wednesday, September 29, 1:45 – 2:45 p.m.
Exhibit Hall, Theater 1
Supported by Thermo Fisher Scientific 

Overview

Most methods now employed to detect viral and bacterial pathogens require a pathogen-rich sample obtained from the infection site. These assays are thus of limited use when the infection site is unknown, inaccessible, or when the responsible pathogen is present at low abundance or exhibits a heterogenous distribution that can lead to sampling errors. Herein we describe a robust and integrated workflow for the identification of pathogen-specific peptides that can be utilized as culture or circulating biomarkers for viral and bacterial infection, and distinguish between highly related pathogens in a single spectrum by target peptide sequence variations to identify species that exhibit different virulence or require different interventions.

Outcomes

After attending this session, participants will be able to:

  • Understand the standard selection criteria for species-specific biomarker peptides that can induce robust antibodies for biomarker immunoprecipitation and which produce strong mass spectrometry signals.
  • Learn how to establish a streamlined workflow that enables high-throughput sample processing with low variation.
  • Learn how to develop a set of criteria to improve the detection of low abundance biomarker peptide peaks among a high dynamic sample background that arises from contaminating ions or electronic noise.

Moderator

Shawnna Donop
Thermo Fisher Scientific

Speaker

Tony Y. Hu, PhD
Prof. Biochemistry & Molecular Biology, Prof. Div. Microbiology, Prof. Biomedical Engineering
Tulane National Primate Research Center
New Orleans, LA